Slash downstream processing costs for producing biologicals with this new chromatography technology
Have You ever heards new technology for downstream processing of biologicals called BioSC (Biopharmaceutical Sequential Chromatography) ? They uses a process known as sequential multi-column continuous chromatography (SMCC), which improves throughput and stationary phase productivity for downstream processing up to four times without compromising product quality or yield, says the company. The technology is suitable for all types of biomolecules, including monoclonal antibodies (mAbs), peptides, blood fractions and vaccines.
SMCC resembles simulated-moving bed(SMB) chromatography in that it is a counter- current process where fluid circulates continuously through a loop of columns, with feed and eluent being added to certain columns, while fractions of interest are recovered sequentially from each column (diagram). The key difference is in SMCC’s flexible scheduling of each of the multiple columns and the ability to asynchronously and flexibly schedule each of the multiple columns, which enables complex, multi-component separations, says Stephen Tingley, vice president of sales and marketing. “This means that each column can simultaneously be working in a different step phase, such as load, wash regeneration and so on.” BioSC overcomes the limitations of conventional batch chromatography, where: the column loading is limited by its dynamic capacity; process flowrates are limited by mechanical stability of the packed bed; and the stationary phase efficiency is limited by saturation of the resin binding sites. “Initial modeling studies of a commercially relevant mAb process clearly indicate the potential of a BioSC-based continuous downstream purification process to reduce the overall capital by up to 30%, equating to a cost of goods reduction of about 49%, with water usage reduced by up to 78% and overall downstream processing costs reduced by up to 69%, says managing director Andrew Sinclair.
SMCC resembles simulated-moving bed(SMB) chromatography in that it is a counter- current process where fluid circulates continuously through a loop of columns, with feed and eluent being added to certain columns, while fractions of interest are recovered sequentially from each column (diagram). The key difference is in SMCC’s flexible scheduling of each of the multiple columns and the ability to asynchronously and flexibly schedule each of the multiple columns, which enables complex, multi-component separations, says Stephen Tingley, vice president of sales and marketing. “This means that each column can simultaneously be working in a different step phase, such as load, wash regeneration and so on.” BioSC overcomes the limitations of conventional batch chromatography, where: the column loading is limited by its dynamic capacity; process flowrates are limited by mechanical stability of the packed bed; and the stationary phase efficiency is limited by saturation of the resin binding sites. “Initial modeling studies of a commercially relevant mAb process clearly indicate the potential of a BioSC-based continuous downstream purification process to reduce the overall capital by up to 30%, equating to a cost of goods reduction of about 49%, with water usage reduced by up to 78% and overall downstream processing costs reduced by up to 69%, says managing director Andrew Sinclair.
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